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Article

Multiplexed Elimination of Wild-Type DNA and High-Resolution Melting Prior to Targeted Resequencing of Liquid Biopsies.

Clinical chemistry - 1 Oct 2017

Ladas Ioannis, Fitarelli-Kiehl Mariana, Song Chen, Adalsteinsson Viktor A, Parsons Heather A, Lin Nancy U, Wagle Nikhil, Makrigiorgos G Mike

Abstract excerpt

BACKGROUND: The use of clinical samples and circulating cell-free DNA (cfDNA) collected from liquid biopsies for diagnostic and prognostic applications in cancer is burgeoning, and improved methods that reduce the influence of excess wild-type (WT) portion of the sample are desirable. Here we present enrichment of mutation-containing sequences using enzymatic degradation of WT DNA. Mutation enrichment is combined...

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Identifiers

PMID: 28679646
DOI: 10.1373/clinchem.2017.272849
PMCID: PMC5914173

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