Article
Human cell based directed evolution of adenine base editors with improved efficiency.
Nature communications - 8 Oct 2021
Fu Junhao, Li Qing, Liu Xiaoyu, Tu Tianxiang, Lv Xiujuan, Yin Xidi, Lv Jineng, Song Zongming, Qu Jia, Zhang Jinwei, Li Jinsong, Gu Feng
Abstract excerpt
Adenine base editors (ABE) are genome-editing tools that have been harnessed to introduce precise A•T to G•C conversion. However, the low activity of ABE at certain sites remains a major bottleneck that precludes efficacious applications. Here, to address it, we develop a directional screening system in human cells to evolve the deaminase component of the ABE, and identify three high-activity NG-ABEmax variants:...
Topics
Join the communities discussing this publication.