Article
Triple base editor catalyzes saturation mutation of adenine, cytidine, and guanine.
Nucleic acids research - 14 Jan 2026
Wu Youming, Wang Jinxin, Zhang Ziyi, Shang Mengyu, Wang Shuping, Li Yinuo, Li Guangyu, Lu Shuangshuang, Ji Kaiyuan, Wang Xiaoyue, Zhang Xiaohui
Abstract excerpt
Current base editors act on a maximum of two base substrates and generate limited base conversions or transversions, hindering their applicability for inducing DNA sequence diversity. Here, we engineered a triple base editor (named ACG-BEs) using a fusion of adenine base editor with high A/C catalytic activity and evolved N-methylpurine DNA glycosylase. ACG-BEs enables efficient, multiplexed saturation...
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