Engineered Campylobacter jejuni Cas9 variant with enhanced activity

Abstract excerpt

<title>Abstract</title> <p>The RNA-guided DNA endonuclease Cas9 is a versatile genome-editing tool. However, the molecular weight of the commonly used Streptococcus pyogenes Cas9 is relatively large. Consequently, its gene cannot be efficiently packaged into an adeno-associated virus vector, thereby limiting its applications for therapeutic genome editing. Here, we biochemically characterized the compact Cas9 fro...

Topics

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• Clustered Regularly Interspaced Short Palindromic Repeats
• CRISPR and Genetic Engineering
• CRISPR-Associated Protein 9
• CRISPR-Cas Systems
• Gene Editing
• Genome
• Mutation
• Streptococcus pyogenes

Identifiers and source

Literature Corpus work

04397cfe-3c67-56a8-a4c1-30cc2ae05ae9

DOI

10.21203/rs.3.rs-512474/v1