Article

Spatial Perturb-Seq: Single-Cell Functional Genomics within Intact Tissue Architecture

2024-12-20

Abstract excerpt

We develop Spatial Perturb-Seq, an in vivo CRISPR technology that interrogates multiple genes within single cells of intact tissues. We apply Spatial Perturb-Seq to screen risk genes for neurodegenerative diseases in the mouse brain, uncovering cell autonomous and cell-cell microenvironmental effects within the spatially intact tissue. Spatial Perturb-Seq functionally screens multiple genes in situ and in vivo...

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Identifiers and source

Literature Corpus work: 31478e5a-1933-5f61-aa54-41fd6579dd7c
DOI: 10.1101/2024.12.19.628843

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