Article

Optimized CRISPR-mediated gene knock-in reveals FOXP3-independent control of human Treg identity

2021-01-17

Abstract excerpt

<h4>Summary</h4> Treg cell therapy is a promising curative approach for a variety of immune-mediated conditions. CRISPR-based genome editing allows precise insertion of transgenes through homology-directed repair, but use in human Tregs has been limited. We report an optimized protocol for CRISPR-mediated gene knock-in in human Tregs with high-yield expansion. To establish a benchmark of human Treg dysfunction, w...

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Literature Corpus work: 1203d271-0852-5a74-bfa8-3135eeb50e65
DOI: 10.1101/2021.01.16.426937

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