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Article

Optimized CRISPR-mediated gene knockin reveals FOXP3-independent maintenance of human Treg identity.

Cell reports - 3 Aug 2021

Lam Avery J, Lin David T S, Gillies Jana K, Uday Prakruti, Pesenacker Anne M, Kobor Michael S, Levings Megan K

Abstract excerpt

Regulatory T cell (Treg) therapy is a promising curative approach for a variety of immune-mediated conditions. CRISPR-based genome editing allows precise insertion of transgenes through homology-directed repair, but its use in human Tregs has been limited. We report an optimized protocol for CRISPR-mediated gene knockin in human Tregs with high-yield expansion. To establish a benchmark of human Treg dysfunction,...

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PMID: 34348163
DOI: 10.1016/j.celrep.2021.109494

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