Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Mutant-Initiated Structure-Guided Refinement Enables Second-Generation Compact IscB Genome Editors.

Chembiochem : a European journal of chemical biology - 27 May 2026

Sha Taixin, Zhao Dongdong, Zhao Xiumei, Lu Yanfang, Wang Rongfei, Liu Jikai, Li Yanrong, Li Siwei, Chen Ming, Bi Changhao, Zhang Xueli

Abstract excerpt

Compact RNA-guided nucleases such as IscB represent an attractive foundation for next-generation genome editors, yet their application in mammalian cells has been constrained by suboptimal activity. Here, instead of re-engineering enzymes, we establish a mutant-initiated, structure-guided optimization strategy to generate second-generation high-activity IscB editors. Using AlphaFold3 to model the engineered...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to CRISPR-Cas Systems
Share to DNA

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Gene Editing

Share to Mutation

Source

Identifiers

PMID: 42178964
DOI: 10.1002/cbic.70392

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Mutant-Initiated Structure-Guided Refinement Enables Second-Generation Compact IscB Genome Editors.PMID 42178964Read ↗

Deep learning-guided engineering of SpuFz1 and rational miniaturization of ωRNA enables efficient genome editing.Semantic proximity Read ↗

Rapid customization of base editors via machine learning-powered combinatorial mutagenesis.Semantic proximity Read ↗

Structure-guided engineering of AI-derived adenine base editors for nuclear and mitochondrial DNA editing.Semantic proximity Read ↗

Structure and evolution-guided design of minimal RNA-guided nucleasesSemantic proximity Read ↗

Enhanced Taq Variant Enables Efficient Genome Editing Testing and Mutation Detection.Semantic proximity Read ↗

Efficient Genome Editing with Chimeric Oligonucleotide-Directed EditingSemantic proximity Read ↗

Accurate inference of the full base-pairing structure of RNA by deep mutational scanning and covariation-induced deviation of activitySemantic proximity Read ↗

Structure-guided discovery and engineering of miniature CRISPR-Cas12m for epigenome editingSemantic proximity Read ↗

Towards optimizing diversifying base editors for high-throughput studies of single- nucleotide variantsSemantic proximity Read ↗

Engineering of a high-fidelity Cas12a nuclease variant capable of allele-specific editing.Semantic proximity Read ↗

Directed evolution of compact RNA-guided nucleases for enhanced activity in mammalian cellsSemantic proximity Read ↗

High-precision cytosine base editors by evolving nucleic-acid-recognition hotspots in deaminase.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.