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Article

A recombineering pipeline for functional genomics applied to Caenorhabditis elegans.

Nature methods - 1 Oct 2006

Sarov Mihail, Schneider Susan, Pozniakovski Andrei, Roguev Assen, Ernst Susanne, Zhang Youming, Hyman A Anthony, Stewart A Francis

Abstract excerpt

We present a new concept in DNA engineering based on a pipeline of serial recombineering steps in liquid culture. This approach is fast, straightforward and facilitates simultaneous processing of multiple samples in parallel. We validated the approach by generating green fluorescent protein (GFP)-tagged transgenes from Caenorhabditis briggsae genomic clones in a multistep pipeline that takes only 4 d. The...

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PMID: 16990816
DOI: 10.1038/nmeth933

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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