Article

Barcoded monoclonal embryoids are a potential solution to confounding bottlenecks in mosaic organoid screens

2025-05-24

Abstract excerpt

Genetic screens in organoids hold tremendous promise for accelerating discoveries at the intersection of genomics and developmental biology. Embryoid bodies (EBs) are self-organizing multicellular structures that recapitulate aspects of early mammalian embryogenesis. We set out to perform a CRISPR screen perturbing all transcription factors (TFs) in murine EBs. Specifically, a library of TF-targeting guide RNAs (g...

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Literature Corpus work: 0decb289-e426-53c7-a76a-24a979e2c971
DOI: 10.1101/2025.05.23.655669

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