Article
Single-cell, whole-embryo phenotyping of mammalian developmental disorders.
Nature - 1 Nov 2023
Huang Xingfan, Henck Jana, Qiu Chengxiang, Sreenivasan Varun K A, Balachandran Saranya, Amarie Oana V, Hrabě de Angelis Martin, Behncke Rose Yinghan, Chan Wing-Lee, Despang Alexandra, Dickel Diane E, Duran Madeleine, Feuchtinger Annette, Fuchs Helmut, Gailus-Durner Valerie, Haag Natja, Hägerling Rene, Hansmeier Nils, Hennig Friederike, Marshall Cooper, Rajderkar Sudha, Ringel Alessa, Robson Michael, Saunders Lauren M, da Silva-Buttkus Patricia, Spielmann Nadine, Srivatsan Sanjay R, Ulferts Sascha, Wittler Lars, Zhu Yiwen, Kalscheuer Vera M, Ibrahim Daniel M, Kurth Ingo, Kornak Uwe, Visel Axel, Pennacchio Len A, Beier David R, Trapnell Cole, Cao Junyue, Shendure Jay, Spielmann Malte
Abstract excerpt
Mouse models are a critical tool for studying human diseases, particularly developmental disorders1. However, conventional approaches for phenotyping may fail to detect subtle defects throughout the developing mouse2. Here we set out to establish single-cell RNA sequencing of the whole embryo as a scalable platform for the systematic phenotyping of mouse genetic models. We applied combinatorial indexing-based...
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