A reversible haploid mouse embryonic stem cell biobank resource for functional genomics.

doi:10.1038/nature24027

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Article

A reversible haploid mouse embryonic stem cell biobank resource for functional genomics.

Nature - 5 Oct 2017

Elling Ulrich, Wimmer Reiner A, Leibbrandt Andreas, Burkard Thomas, Michlits Georg, Leopoldi Alexandra, Micheler Thomas, Abdeen Dana, Zhuk Sergei, Aspalter Irene M, Handl Cornelia, Liebergesell Julia, Hubmann Maria, Husa Anna-Maria, Kinzer Manuela, Schuller Nicole, Wetzel Ellen, van de Loo Nina, Martinez Jorge Arturo Zepeda, Estoppey David, Riedl Ralph, Yang Fengtang, Fu Beiyuan, Dechat Thomas, Ivics Zoltán, Agu Chukwuma A, Bell Oliver, Blaas Dieter, Gerhardt Holger, Hoepfner Dominic, Stark Alexander, Penninger Josef M

Abstract excerpt

The ability to directly uncover the contributions of genes to a given phenotype is fundamental for biology research. However, ostensibly homogeneous cell populations exhibit large clonal variance that can confound analyses and undermine reproducibility. Here we used genome-saturated mutagenesis to create a biobank of over 100,000 individual haploid mouse embryonic stem (mES) cell lines targeting 16,970 genes with...

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PMID: 28953874
DOI: 10.1038/nature24027
PMCID: PMC6235111

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