Article

Transcriptomics-informed pharmacology identifies epigenetic and cell cycle regulators as enhancers of AAV production

2024-06-15

Abstract excerpt

Recombinant adeno-associated virus (rAAV) is a widely used viral vector for gene therapy. Despite its clinical efficacy, the manufacturing of rAAV faces challenges in productivity and quality, leading to limited availability. To address the growing demand, next-generation process development should be informed by a mechanistic understanding of the cellular response to rAAV. In this study, we performed transcriptom...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 0aa52e45-efcd-5cbf-b4e1-7fb6dc3a9834
DOI: 10.1101/2024.06.14.599118

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Transcriptomics-informed pharmacology identifies epigenetic and cell cycle regulators as enhancers of AAV productionDOI 10.1101/2024.06.14.599118Read

Advancing AAV production with high-throughput screening and transcriptomicsSemantic proximity Read

Production of rAAV by plasmid transfection induces antiviral and inflammatory responses in suspension HEK293 cellsSemantic proximity Read

Analytical methods for process and product characterization of recombinant adeno-associated virus-based gene therapiesSemantic proximity Read

Genome-wide activation screens to increase adeno-associated virus productionSemantic proximity Read

AAV Assembled Capsids Are Produced in Cells Blocked from Cell Cycle Progression.Semantic proximity Read

Proteomic Landscape of Adeno-Associated Virus (AAV)-Producing HEK293 CellsSemantic proximity Read

Enhanced ER protein processing gene expression increases rAAV yield and full capsid ratio in HEK293 cellsSemantic proximity Read

Increased recombinant AAV production by HEK293 cells using small molecule chemical additivesSemantic proximity Read

Recent advances in upstream process development for production of recombinant adeno‐associated virusSemantic proximity Read

Molecular design for recombinant adeno-associated virus (rAAV) vector productionSemantic proximity Read

Enhancement of rAAV titers via inhibition of the interferon signaling cascade in transfected HEK293 suspension culturesSemantic proximity Read

Transcriptomic functional characterization of recombinant adeno‐associated virus producing cell line adapted to suspension‐growthSemantic proximity Read