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Article

Genome-wide activation screens to increase adeno-associated virus production

16 Jul 2021

Abstract excerpt

We describe a genome-wide screening strategy to identify target genes whose modulation increases the capacity of a cell to produce recombinant adeno-associated viral (AAV) vector. Specifically, a single-guide RNA (sgRNA) library for a CRISPR-based genome-wide transcriptional activation screen was inserted into an AAV vector, and iterative rounds of viral infection and rescue in HEK293 producer cells enabled the...

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PMID: 34513296
DOI: 10.1016/j.omtn.2021.06.026

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