Article
A novel approach to quantify G-protein-coupled receptor dimerization equilibrium using bioluminescence resonance energy transfer.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013
Kufareva Irina, Stephens Bryan, Gilliland C Taylor, Wu Beili, Fenalti Gustavo, Hamel Damon, Stevens Raymond C, Abagyan Ruben, Handel Tracy M
Abstract excerpt
Along with other resonance energy transfer techniques, bioluminescence resonance energy transfer (BRET) has emerged as an important method for demonstrating protein-protein interactions in cells. In the field of G-protein-coupled receptors, including chemokine receptors, BRET has been widely used to investigate homo- and heterodimerization, a feature of their interactions that is emerging as integral to function...
Topics
- Animals
- Bioluminescence Resonance Energy Transfer Techniques
- Cells, Cultured
- Humans
- Models, Molecular
- Mutation
- Protein Conformation
- Protein Multimerization
- Receptors, CXCR4
- Receptors, G-Protein-Coupled