Article
A novel approach to quantify G-protein-coupled receptor dimerization equilibrium using bioluminescence resonance energy transfer.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013
Kufareva Irina, Stephens Bryan, Gilliland C Taylor, Wu Beili, Fenalti Gustavo, Hamel Damon, Stevens Raymond C, Abagyan Ruben, Handel Tracy M
Abstract excerpt
Along with other resonance energy transfer techniques, bioluminescence resonance energy transfer (BRET) has emerged as an important method for demonstrating protein-protein interactions in cells. In the field of G-protein-coupled receptors, including chemokine receptors, BRET has been widely used to investigate homo- and heterodimerization, a feature of their interactions that is emerging as integral to function...
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