Article

Protocol for measuring endocrine disruptive effects on transcriptional bursting using single-molecule imaging in human breast cancer cells

2026-05-05

Abstract excerpt

<h4>SUMMARY</h4> Transcriptional bursts regulate gene expression by altering burst size or burst frequency. Here, we present a protocol that integrates fixed-cell smFISH and live-cell single-molecule imaging to analyze estrogen-responsive transcriptional bursting of the TFF1 gene in human breast cancer cell lines. This workflow enables measurement of burst size, burst initiation, and active allele frequency to de...

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Literature Corpus work: 078801eb-fa9c-5170-96e2-909304796454
DOI: 10.64898/2026.05.01.722245

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