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Article

Single-allele analysis of transcription kinetics in living mammalian cells.

Nature methods - 1 Aug 2010

Yunger Sharon, Rosenfeld Liat, Garini Yuval, Shav-Tal Yaron

Abstract excerpt

We generated a system for in vivo visualization and analysis of mammalian mRNA transcriptional kinetics of single alleles in real time, using single-gene integrations. We obtained high-resolution transcription measurements of a single cyclin D1 allele under endogenous or viral promoter control, including quantification of temporal kinetics of transcriptional bursting, promoter firing, nascent mRNA numbers and...

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PMID: 20639867
DOI: 10.1038/nmeth.1482

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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