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Article

TrfA dimers play a role in copy-number control of RK2 replication.

Gene - 26 Nov 1998

Toukdarian A E, Helinski D R

Abstract excerpt

Copy-number regulation of the broad-host-range plasmid RK2 is dependent on the plasmid-encoded initiator protein, TrfA, and the RK2 origin of replication. The handcuffing model for copy-number control proposes that TrfA-bound oris reversibly couple to prevent the further initiation of plasmid replication when the copy number in vivo is at or above the replicon-specific copy number. TrfA mutants have been isolated...

Topics

Bacterial Proteins
DNA Replication
DNA, Bacterial
Dimerization
Escherichia coli Proteins
Gene Dosage
Genes, Suppressor
Mutation
Phenotype
Plasmids
Replication Origin

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 9858734
DOI: 10.1016/s0378-1119(98)00370-9

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TrfA dimers play a role in copy-number control of RK2 replication.PMID 9858734Read ↗

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