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Article

Iteron inhibition of plasmid RK2 replication in vitro: evidence for intermolecular coupling of replication origins as a mechanism for RK2 replication control.

Proceedings of the National Academy of Sciences of the United States of America - 15 Feb 1991

Kittell B L, Helinski D R

Abstract excerpt

The broad-host-range plasmid RK2 and its derivatives are maintained in Gram-negative bacteria at a specific copy number that appears to be determined by a series of direct repeats (iterons) located at the RK2 replication origin and by the RK2 replication initiation protein. TrfA. An in vitro replication system was developed from Escherichia coli that is active with either the intact eight-iteron RK2 origin or a...

Topics

Bacterial Proteins
DNA Replication
Escherichia coli
Escherichia coli Proteins
Kinetics
Mutation
Plasmids
Repetitive Sequences, Nucleic Acid
Restriction Mapping

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Identifiers

PMID: 1996339
DOI: 10.1073/pnas.88.4.1389
PMCID: PMC51023

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1996339-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Iteron inhibition of plasmid RK2 replication in vitro: evidence for intermolecular coupling of replication origins as a mechanism for RK2 replication control.PMID 1996339Read ↗

Copy-up mutants of the plasmid RK2 replication initiation protein are defective in coupling RK2 replication origins.Semantic proximity Read ↗

TrfA dimers play a role in copy-number control of RK2 replication.Semantic proximity Read ↗

Mutations in the gene encoding the replication-initiation protein of plasmid RK2 produce elevated copy numbers of RK2 derivatives in Escherichia coli and distantly related bacteria.Semantic proximity Read ↗

Mutations in the trfA replication gene of the broad-host-range plasmid RK2 result in elevated plasmid copy numbers.Semantic proximity Read ↗

Replication origin of the broad host range plasmid RK2. Positioning of various motifs is critical for initiation of replication.Semantic proximity Read ↗

The phenotypes of temperature-sensitive mini-RK2 replicons carrying mutations in the replication control gene trfA are suppressed nonspecifically by intragenic cop mutations.Semantic proximity Read ↗

Positioning and the specific sequence of each 13-mer motif are critical for activity of the plasmid RK2 replication origin.Semantic proximity Read ↗

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Control of plasmid replication—How do DNA iterons set the replication frequency?Semantic proximity Read ↗

The host range of RK2 minimal replicon copy-up mutants is limited by species-specific differences in the maximum tolerable copy number.Semantic proximity Read ↗

Mechanism of origin activation by monomers of R6K-encoded pi protein.Semantic proximity Read ↗

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