Mutations in the gene encoding the replication-initiation protein of plasmid RK2 produce elevated copy numbers of RK2 derivatives in Escherichia coli and distantly related bacteria.

doi:10.1016/0378-1119(93)90217-q

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Article

Mutations in the gene encoding the replication-initiation protein of plasmid RK2 produce elevated copy numbers of RK2 derivatives in Escherichia coli and distantly related bacteria.

Gene - 29 Oct 1993

Fang F C, Durland R H, Helinski D R

Abstract excerpt

Mini-replicons of the broad-host-range plasmid RK2 with increased copy number (cn) due to mutations in the gene encoding the essential replication initiation protein TrfA are described. The cn of these derivatives have been determined in Escherichia coli, Pseudomonas aeruginosa and Agrobacterium...

Topics

Bacterial Proteins
Blotting, Western
Cloning, Molecular
DNA Replication
Escherichia coli
Escherichia coli Proteins
Gram-Negative Bacteria
Mutation
Plasmids
Restriction Mapping

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