Article
The phenotypes of temperature-sensitive mini-RK2 replicons carrying mutations in the replication control gene trfA are suppressed nonspecifically by intragenic cop mutations.
Journal of bacteriology - 1 Nov 1992
Haugan K, Karunakaran P, Blatny J M, Valla S
Abstract excerpt
The minimal replicon of the broad-host-range plasmid RK2 consists of the origin of vegetative replication (oriV) and a gene (trfA) encoding an essential replication protein that binds to short repeats in oriV. We report here the results of a DNA sequence analysis of seven unique mutants that are...
Topics
- Bacterial Proteins
- Base Sequence
- Chromosome Mapping
- Escherichia coli
- Escherichia coli Proteins
- Genes, Bacterial
- Genes, Regulator
- Hot Temperature
- Molecular Sequence Data
- Mutation
- Phenotype
- Plasmids
- Pseudomonas aeruginosa
- Regulatory Sequences, Nucleic Acid