The host range of RK2 minimal replicon copy-up mutants is limited by species-specific differences in the maximum tolerable copy number.

doi:10.1006/plas.1995.1004

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Article

The host range of RK2 minimal replicon copy-up mutants is limited by species-specific differences in the maximum tolerable copy number.

Plasmid - 1 Jan 1995

Haugan K, Karunakaran P, Tøndervik A, Valla S

Abstract excerpt

The minimal replicon of the broad-host-range plasmid RK2 consists of a gene, trfA (trans-acting replication), encoding a protein required for initiation of plasmid replication. The TrfA protein binds to iterons in the cis-acting origin of vegetative replication (oriV), but the exact mechanism by which TrfA-mediated replication initiation takes place is not known. We report here the isolation and characterization...

Topics

Ampicillin
Azotobacter vinelandii
Bacteria
Bacterial Proteins
Conjugation, Genetic
DNA Replication
Escherichia coli
Escherichia coli Proteins
Microbial Sensitivity Tests
Mutagenesis
Phenotype

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Plasmids

Pseudomonas aeruginosa

Replicon

Restriction Mapping

Salmonella typhimurium

Transformation, Bacterial

Source

Open in PubMed

Identifiers

PMID: 7753906
DOI: 10.1006/plas.1995.1004

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7753906-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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The host range of RK2 minimal replicon copy-up mutants is limited by species-specific differences in the maximum tolerable copy number.PMID 7753906Read ↗

Mutations in the gene encoding the replication-initiation protein of plasmid RK2 produce elevated copy numbers of RK2 derivatives in Escherichia coli and distantly related bacteria.Semantic proximity Read ↗

The phenotypes of temperature-sensitive mini-RK2 replicons carrying mutations in the replication control gene trfA are suppressed nonspecifically by intragenic cop mutations.Semantic proximity Read ↗

Mutations in the trfA replication gene of the broad-host-range plasmid RK2 result in elevated plasmid copy numbers.Semantic proximity Read ↗

TrfA dimers play a role in copy-number control of RK2 replication.Semantic proximity Read ↗

Copy-up mutants of the plasmid RK2 replication initiation protein are defective in coupling RK2 replication origins.Semantic proximity Read ↗

Host-dependent requirement for specific DnaA boxes for plasmid RK2 replication.Semantic proximity Read ↗

Iteron inhibition of plasmid RK2 replication in vitro: evidence for intermolecular coupling of replication origins as a mechanism for RK2 replication control.Semantic proximity Read ↗

A region of the broad-host-range plasmid RK2 causes stable in planta inheritance of plasmids in Rhizobium meliloti cells isolated from alfalfa root nodules.Semantic proximity Read ↗

Species-dependent phenotypes of replication-temperature-sensitive trfA mutants of plasmid RK2: a codon-neutral base substitution stimulates temperature sensitivity by leading to reduced levels of trfA expression.Semantic proximity Read ↗

Positioning and the specific sequence of each 13-mer motif are critical for activity of the plasmid RK2 replication origin.Semantic proximity Read ↗

Eclipse period during replication of plasmid R1: contributions from structural events and from the copy-number control system.Semantic proximity Read ↗

Modulation of pPS10 host range by plasmid-encoded RepA initiator protein.Semantic proximity Read ↗

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