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Article

Positioning and the specific sequence of each 13-mer motif are critical for activity of the plasmid RK2 replication origin.

Molecular microbiology - 1 Sept 2005

Kowalczyk Lukasz, Rajewska Magdalena, Konieczny Igor

Abstract excerpt

The minimal replication origin of the broad-host-range plasmid RK2, oriV, contains five iterons which are binding sites for the plasmid-encoded replication initiation protein TrfA, four DnaA boxes, which bind the host DnaA protein, and an AT-rich region containing four 13-mer sequences. In this study, 26 mutants with altered sequence and/or spacing of 13-mer motifs have been constructed and analysed for...

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Identifiers

PMID: 16102011
DOI: 10.1111/j.1365-2958.2005.04770.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Positioning and the specific sequence of each 13-mer motif are critical for activity of the plasmid RK2 replication origin.PMID 16102011Read ↗

Replication origin of the broad host range plasmid RK2. Positioning of various motifs is critical for initiation of replication.Semantic proximity Read ↗

Specific mutations within the AT-rich region of a plasmid replication origin affect either origin opening or helicase loading.Semantic proximity Read ↗

Host-dependent requirement for specific DnaA boxes for plasmid RK2 replication.Semantic proximity Read ↗

Copy-up mutants of the plasmid RK2 replication initiation protein are defective in coupling RK2 replication origins.Semantic proximity Read ↗

A critical DnaA box directs the cooperative binding of the Escherichia coli DnaA protein to the plasmid RK2 replication origin.Semantic proximity Read ↗

Iteron inhibition of plasmid RK2 replication in vitro: evidence for intermolecular coupling of replication origins as a mechanism for RK2 replication control.Semantic proximity Read ↗

The box VII motif of Escherichia coli DnaA protein is required for DnaA oligomerization at the E. coli replication origin.Semantic proximity Read ↗

A single DnaA box is sufficient for initiation from the P1 plasmid origin.Semantic proximity Read ↗

A comprehensive set of DnaA-box mutations in the replication origin, oriC, of Escherichia coli.Semantic proximity Read ↗

The DnaA box R4 in the minimal oriC is dispensable for initiation of Escherichia coli chromosome replication.Semantic proximity Read ↗

A specific region in the N terminus of a replication initiation protein of plasmid RK2 is required for recruitment of Pseudomonas aeruginosa DnaB helicase to the plasmid origin.Semantic proximity Read ↗

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