Article
Copy-up mutants of the plasmid RK2 replication initiation protein are defective in coupling RK2 replication origins.
Proceedings of the National Academy of Sciences of the United States of America - 16 Apr 1996
Blasina A, Kittell B L, Toukdarian A E, Helinski D R
Abstract excerpt
The broad host range plasmid RK2 replicates and regulates its copy number in a wide range of Gram-negative bacteria. The plasmid-encoded trans-acting replication protein TrfA and the origin of replication oriV are sufficient for controlled replication of the plasmid in all Gram-negative bacteria...
Topics
- Bacterial Proteins
- Base Sequence
- DNA, Bacterial
- Escherichia coli
- Escherichia coli Proteins
- Gene Amplification
- Genes, Bacterial
- Gram-Negative Bacteria
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Plasmids
- Repetitive Sequences, Nucleic Acid
- Replication Origin
- Transformation, Genetic