Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

The replication initiator operon of promiscuous plasmid RK2 encodes a gene that complements an Escherichia coli mutant defective in single-stranded DNA-binding protein.

Journal of bacteriology - 1 Jul 1992

Jovanovic O S, Ayres E K, Figurski D H

Abstract excerpt

The amino acid sequence of the 13-kDa polypeptide (P116) encoded by the first gene of the trfA operon of IncP plasmid RK2 shows significant similarity to several known single-stranded DNA-binding proteins. We found that unregulated expression of this gene from its natural promoter (trfAp) or indu...

Topics

Amino Acid Sequence
Bacterial Proteins
Base Sequence
DNA Replication
DNA-Binding Proteins
Escherichia coli
Escherichia coli Proteins
Genetic Complementation Test
Molecular Sequence Data
Mutation
Operon

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Plasmids

Promoter Regions, Genetic

Temperature

Source

Open in PubMed Open full text

Identifiers

PMID: 1624472
DOI: 10.1128/jb.174.14.4842-4846.1992
PMCID: PMC206285

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1624472-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

The replication initiator operon of promiscuous plasmid RK2 encodes a gene that complements an Escherichia coli mutant defective in single-stranded DNA-binding protein.PMID 1624472Read ↗

Mutations in the trfA replication gene of the broad-host-range plasmid RK2 result in elevated plasmid copy numbers.Semantic proximity Read ↗

Mutations in the gene encoding the replication-initiation protein of plasmid RK2 produce elevated copy numbers of RK2 derivatives in Escherichia coli and distantly related bacteria.Semantic proximity Read ↗

A specific region in the N terminus of a replication initiation protein of plasmid RK2 is required for recruitment of Pseudomonas aeruginosa DnaB helicase to the plasmid origin.Semantic proximity Read ↗

Species-dependent phenotypes of replication-temperature-sensitive trfA mutants of plasmid RK2: a codon-neutral base substitution stimulates temperature sensitivity by leading to reduced levels of trfA expression.Semantic proximity Read ↗

Conjugative transfer functions of broad-host-range plasmid RK2 are coregulated with vegetative replication.Semantic proximity Read ↗

Copy-up mutants of the plasmid RK2 replication initiation protein are defective in coupling RK2 replication origins.Semantic proximity Read ↗

TrfA dimers play a role in copy-number control of RK2 replication.Semantic proximity Read ↗

The phenotypes of temperature-sensitive mini-RK2 replicons carrying mutations in the replication control gene trfA are suppressed nonspecifically by intragenic cop mutations.Semantic proximity Read ↗

Identification of a potential membrane-targeting region of the replication initiator protein (TrfA) of broad-host-range plasmid RK2.Semantic proximity Read ↗

Identification of a maintenance system on rolling circle replicating plasmid pVT736-1. dgalli@iusd.iupui.edu.Semantic proximity Read ↗

Molecular analysis and characterization of a broad-host-range plasmid, pEP2.Semantic proximity Read ↗

Characterization of the minimum replicon of the broad-host-range plasmid pTF-FC2 and similarity between pTF-FC2 and the IncQ plasmids.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.