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Article

The replication initiator operon of promiscuous plasmid RK2 encodes a gene that complements an Escherichia coli mutant defective in single-stranded DNA-binding protein.

Journal of bacteriology - 1 Jul 1992

Jovanovic O S, Ayres E K, Figurski D H

Abstract excerpt

The amino acid sequence of the 13-kDa polypeptide (P116) encoded by the first gene of the trfA operon of IncP plasmid RK2 shows significant similarity to several known single-stranded DNA-binding proteins. We found that unregulated expression of this gene from its natural promoter (trfAp) or induced expression from a strong heterologous promoter (trcp) was sufficient to complement the temperature-sensitive growth...

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Identifiers

PMID: 1624472
DOI: 10.1128/jb.174.14.4842-4846.1992
PMCID: PMC206285

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Source receipt: pubmed, retrieved 7 Sept 2026.

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The replication initiator operon of promiscuous plasmid RK2 encodes a gene that complements an Escherichia coli mutant defective in single-stranded DNA-binding protein.PMID 1624472Read ↗

Mutations in the trfA replication gene of the broad-host-range plasmid RK2 result in elevated plasmid copy numbers.Semantic proximity Read ↗

Mutations in the gene encoding the replication-initiation protein of plasmid RK2 produce elevated copy numbers of RK2 derivatives in Escherichia coli and distantly related bacteria.Semantic proximity Read ↗

A specific region in the N terminus of a replication initiation protein of plasmid RK2 is required for recruitment of Pseudomonas aeruginosa DnaB helicase to the plasmid origin.Semantic proximity Read ↗

Species-dependent phenotypes of replication-temperature-sensitive trfA mutants of plasmid RK2: a codon-neutral base substitution stimulates temperature sensitivity by leading to reduced levels of trfA expression.Semantic proximity Read ↗

Conjugative transfer functions of broad-host-range plasmid RK2 are coregulated with vegetative replication.Semantic proximity Read ↗

Copy-up mutants of the plasmid RK2 replication initiation protein are defective in coupling RK2 replication origins.Semantic proximity Read ↗

TrfA dimers play a role in copy-number control of RK2 replication.Semantic proximity Read ↗

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Identification of a potential membrane-targeting region of the replication initiator protein (TrfA) of broad-host-range plasmid RK2.Semantic proximity Read ↗

Identification of a maintenance system on rolling circle replicating plasmid pVT736-1. dgalli@iusd.iupui.edu.Semantic proximity Read ↗

Molecular analysis and characterization of a broad-host-range plasmid, pEP2.Semantic proximity Read ↗

Characterization of the minimum replicon of the broad-host-range plasmid pTF-FC2 and similarity between pTF-FC2 and the IncQ plasmids.Semantic proximity Read ↗

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