Mutations in the trfA replication gene of the broad-host-range plasmid RK2 result in elevated plasmid copy numbers.

Abstract excerpt

Mutated forms of trfA, the replication protein gene of plasmid RK2, that support a minimal RK2 origin plasmid in Escherichia coli at copy numbers up to 23-fold higher than normal have been isolated. Six such high-copy-number (copy-up) mutations were mapped and sequenced. In each case, a single base transition led to an amino acid substitution in the TrfA protein primary sequence. The six mutations affected...

Topics

• Alleles
• Amino Acid Sequence
• Bacterial Proteins
• Base Sequence
• Escherichia coli
• Genes, Bacterial
• Molecular Sequence Data
• Mutation
• Plasmids
• Restriction Mapping