Article
Disruption of the talin gene compromises focal adhesion assembly in undifferentiated but not differentiated embryonic stem cells.
The Journal of cell biology - 24 Aug 1998
Priddle H, Hemmings L, Monkley S, Woods A, Patel B, Sutton D, Dunn G A, Zicha D, Critchley D R
Abstract excerpt
We have used gene disruption to isolate two talin (-/-) ES cell mutants that contain no intact talin. The undifferentiated cells (a) were unable to spread on gelatin or laminin and grew as rounded colonies, although they were able to spread on fibronectin (b) showed reduced adhesion to laminin, b...
Topics
- Actins
- Animals
- Cell Adhesion
- Cell Differentiation
- Cell Division
- Cinnamates
- Cytoskeletal Proteins
- Extracellular Matrix
- Fibronectins
- Fluorescent Antibody Technique
- Gelatin
- Gene Targeting
- Hygromycin B
- Integrins
- Laminin