Specificity in transcriptional regulation in the absence of specific DNA binding sites: the case of T7 lysozyme.

Abstract excerpt

The binding of T7 lysozyme to T7 RNAP increases the apparent Km for NTP during initiation (formation of the first phosphodiester bond). It also increases the dissociation constant and dissociation rate of product dinucleotide from the polymerase. Higher NTP concentrations are required for maximal...

Topics

• Binding Sites
• DNA, Viral
• DNA-Binding Proteins
• DNA-Directed RNA Polymerases
• Deoxyribonucleases, Type II Site-Specific
• Enzyme Inhibitors
• Gene Expression Regulation
• Guanosine Triphosphate
• Models, Molecular
• Mutation
• N-Acetylmuramoyl-L-alanine Amidase
• Promoter Regions, Genetic
• Protein Conformation
• Ribonucleotides
• Transcription, Genetic
• Viral Proteins