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Article

Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants.

Biochemistry - 29 Aug 2000

Imburgio D, Rong M, Ma K, McAllister W T

Abstract excerpt

We have examined the behavior of T7 RNA polymerase (RNAP) at a set of promoter variants having all possible single base pair (bp) substitutions. The polymerase exhibits an absolute requirement for initiation with a purine and a strong preference for initiation with GTP vs ATP. Promoter variants that would require initiation at the normal start site (+1) with CTP or UTP result in a shift in initiation to +2 (with...

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PMID: 10956032
DOI: 10.1021/bi000365w

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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