Article
The low processivity of T7 RNA polymerase over the initially transcribed sequence can limit productive initiation in vivo.
Journal of molecular biology - 30 May 1997
Lopez P J, Guillerez J, Sousa R, Dreyfus M
Abstract excerpt
In vitro, after binding to the promoter to form a catalytically active complex, RNA polymerases abortively cycle over the first transcribed nucleotides (initial transcribed sequence or ITS) before leaving the promoter. With the bacteriophage T7 enzyme, the extent of abortive transcription varies...
Topics
- Base Sequence
- Capsid
- Capsid Proteins
- DNA-Directed RNA Polymerases
- Escherichia coli
- Molecular Sequence Data
- Mutation
- Plasmids
- Promoter Regions, Genetic
- RNA, Transfer, Arg
- Transcription, Genetic
- Viral Proteins
- beta-Galactosidase