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Article

Promoter specificity determinants of T7 RNA polymerase.

Proceedings of the National Academy of Sciences of the United States of America - 20 Jan 1998

Rong M, He B, McAllister W T, Durbin R K

Abstract excerpt

The high specificity of T7 RNA polymerase (RNAP) for its promoter sequence is mediated, in part, by a specificity loop (residues 742-773) that projects into the DNA binding cleft (1). Previous work demonstrated a role for the amino acid residue at position 748 (N748) in this loop in discrimination of the base pairs (bp) at positions -10 and -11 (2). A comparison of the sequences of other phage RNAPs and their...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 9435223
DOI: 10.1073/pnas.95.2.515
PMCID: PMC18451

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Source receipt: pubmed, retrieved 7 Sept 2026.

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