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Mutation detection and single-molecule counting using isothermal rolling-circle amplification.

Nature genetics - 1 Jul 1998

Lizardi P M, Huang X, Zhu Z, Bray-Ward P, Thomas D C, Ward D C

Abstract excerpt

Rolling-circle amplification (RCA) driven by DNA polymerase can replicate circularized oligonucleotide probes with either linear or geometric kinetics under isothermal conditions. In the presence of two primers, one hybridizing to the + strand, and the other, to the - strand of DNA, a complex pattern of DNA strand displacement ensues that generates 10(9) or more copies of each circle in 90 minutes, enabling...

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PMID: 9662393
DOI: 10.1038/898

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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