Article
Mutation detection and single-molecule counting using isothermal rolling-circle amplification.
Nature genetics - 1 Jul 1998
Lizardi P M, Huang X, Zhu Z, Bray-Ward P, Thomas D C, Ward D C
Abstract excerpt
Rolling-circle amplification (RCA) driven by DNA polymerase can replicate circularized oligonucleotide probes with either linear or geometric kinetics under isothermal conditions. In the presence of two primers, one hybridizing to the + strand, and the other, to the - strand of DNA, a complex pat...
Topics
- Alleles
- Base Sequence
- Cell Line
- Cystic Fibrosis Transmembrane Conductance Regulator
- DNA Mutational Analysis
- DNA Probes
- DNA, Circular
- Humans
- Molecular Sequence Data
- Nucleic Acid Amplification Techniques
- Point Mutation