Article
In situ genotyping individual DNA molecules by target-primed rolling-circle amplification of padlock probes.
Nature methods - 1 Dec 2004
Larsson Chatarina, Koch Jørn, Nygren Anders, Janssen George, Raap Anton K, Landegren Ulf, Nilsson Mats
Abstract excerpt
Methods are needed to study single molecules to reveal variability, interactions and mechanisms that may go undetected at the level of populations of molecules. We describe here an integrated series of reaction steps that allow individual nucleic acid molecules to be detected with excellent specificity. Oligonucleotide probes are circularized after hybridization to target sequences that have been prepared so that...
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