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In Situ Single-Molecule RNA Genotyping Using Padlock Probes and Rolling Circle Amplification.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017

Krzywkowski Tomasz, Hauling Thomas, Nilsson Mats

Abstract excerpt

Present-day techniques allow for massively parallel and high-throughput characterization of the somatic mutation status of samples. Most of these assays rely on whole specimen extracts, where heterogeneous spatial context of the specimen is lost. This chapter describes an up-to-date protocol for multiplexed, in situ genotyping of RNA in preserved tissue and cell lines, using padlock probes and rolling circle...

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PMID: 27822856
DOI: 10.1007/978-1-4939-6442-0_4

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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In Situ Single-Molecule RNA Genotyping Using Padlock Probes and Rolling Circle Amplification.PMID 27822856Read ↗

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Lock and roll: single-molecule genotyping in situ using padlock probes and rolling-circle amplification.Semantic proximity Read ↗

Rolling Circle Amplification With Hairpin Probes for In Situ Single-Molecule RNA Imaging at Single-Base Resolution.Semantic proximity Read ↗

PCR-generated padlock probes detect single nucleotide variation in genomic DNA.Semantic proximity Read ↗

In situ genotyping individual DNA molecules by target-primed rolling-circle amplification of padlock probes.Semantic proximity Read ↗

Analyzing genes using closing and replicating circles.Semantic proximity Read ↗

Imaging of individual transcripts by amplification-based single-molecule fluorescence in situ hybridization.Semantic proximity Read ↗

Mutation detection and single-molecule counting using isothermal rolling-circle amplification.Semantic proximity Read ↗

Ligation-mediated rolling-circle amplification-based approaches to single nucleotide polymorphism detection.Semantic proximity Read ↗

Parallel gene analysis with allele-specific padlock probes and tag microarrays.Semantic proximity Read ↗

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