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Sensitive and inexpensive digital DNA analysis by microfluidic enrichment of rolling circle amplified single-molecules.

Nucleic acids research - 5 May 2017

Kühnemund Malte, Hernández-Neuta Iván, Sharif Mohd Istiaq, Cornaglia Matteo, Gijs Martin A M, Nilsson Mats

Abstract excerpt

Single molecule quantification assays provide the ultimate sensitivity and precision for molecular analysis. However, most digital analysis techniques, i.e. droplet PCR, require sophisticated and expensive instrumentation for molecule compartmentalization, amplification and analysis. Rolling circle amplification (RCA) provides a simpler means for digital analysis. Nevertheless, the sensitivity of RCA assays has...

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PMID: 28077562
DOI: 10.1093/nar/gkw1324
PMCID: PMC5416848

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