Article
Colorimetric detection of single-nucleotide mutations based on rolling circle amplification and G-quadruplex-based DNAzyme.
Analytical methods : advancing methods and applications - 29 Aug 2024
Ouedraogo Serge Yannick, Zeye Moutanou Modeste Judes, Zhou Xi, Kiendrebeogo Touwendpoulimdé Isabelle, Zoure Abdou Azaque, Chen Hanchun, Chen Fangzhi, Ma Changbei
Abstract excerpt
In this work, we proposed a sensitive and selective colorimetric assay for single nucleotide mutation (SNM) detection combining rolling circle amplification (RCA) and G-quadruplex/hemin DNAzyme complex formation. In the detection principle, the first step involves ssDNA hybridization with a padlock probe (PLP) DNA, which can discriminate a single base mismatch. The successful ligation is followed by an RCA event...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.