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Article

Linear amplicons as precursors of amplified circles in methotrexate-resistant Leishmania tarentolae.

Nucleic acids research - 15 Jul 1998

Grondin K, Kündig C, Roy G, Ouellette M

Abstract excerpt

Gene amplification is frequently observed in Leishmania cells selected for drug resistance. By gene targeting we have tagged both alleles of the H locus of Leishmania tarentolae with the neomycin and hygromycin phosphotransferase genes ( neo and hyg ). Selection of these recombinant parasites for low level methotrexate resistance led to amplification of the H locus as part of linear amplicons. The availability of...

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Identifiers

PMID: 9649621
DOI: 10.1093/nar/26.14.3372
PMCID: PMC147699

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Source receipt: pubmed, retrieved 7 Sept 2026.

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