Article
Double targeted gene replacement for creating null mutants.
Proceedings of the National Academy of Sciences of the United States of America - 15 Aug 1991
Cruz A, Coburn C M, Beverley S M
Abstract excerpt
We have used double gene targeting to create homozygous gene replacements in the protozoan parasite Leishmania major, an asexual diploid. This method uses two independent selectable markers in successive rounds of gene targeting to replace both alleles of an endogenous gene. We developed an impro...
Topics
- Alleles
- Animals
- Base Sequence
- Blotting, Southern
- Cell Line
- Diploidy
- Genetic Engineering
- Genetic Markers
- Kanamycin Kinase
- Karyotyping
- Leishmania tropica
- Molecular Sequence Data
- Mutagenesis, Insertional
- Oligonucleotide Probes
- Phosphotransferases
- Phosphotransferases (Alcohol Group Acceptor)
- Polymerase Chain Reaction
- Restriction Mapping