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Article

Double targeted gene replacement for creating null mutants.

Proceedings of the National Academy of Sciences of the United States of America - 15 Aug 1991

Cruz A, Coburn C M, Beverley S M

Abstract excerpt

We have used double gene targeting to create homozygous gene replacements in the protozoan parasite Leishmania major, an asexual diploid. This method uses two independent selectable markers in successive rounds of gene targeting to replace both alleles of an endogenous gene. We developed an improved hygromycin B-resistance cassette encoding hygromycin phosphotransferase (HYG) for use as a selectable marker for...

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Identifiers

PMID: 1651496
DOI: 10.1073/pnas.88.16.7170
PMCID: PMC52255

Genes

DHFR

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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