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Article

Plasticity in chromosome number and testing of essential genes in Leishmania by targeting.

Proceedings of the National Academy of Sciences of the United States of America - 15 Feb 1993

Cruz A K, Titus R, Beverley S M

Abstract excerpt

We attempted to generate homozygous dhfr-ts (dihydrofolate reductase-thymidylate synthase) knockouts in virulent Leishmania major, an asexual diploid protozoan parasite. Transfection of a neo (neomycin phosphotransferase) targeting fragment yielded heterozygous replacement lines with high efficiency. However, second transfections with a hyg (hygromycin B phosphotransferase) targeting fragment in the presence of...

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Identifiers

PMID: 8381972
DOI: 10.1073/pnas.90.4.1599
PMCID: PMC45922

Genes

DHFR

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Source receipt: pubmed, retrieved 7 Sept 2026.

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