Plasticity in chromosome number and testing of essential genes in Leishmania by targeting.

doi:10.1073/pnas.90.4.1599

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Article

Plasticity in chromosome number and testing of essential genes in Leishmania by targeting.

Proceedings of the National Academy of Sciences of the United States of America - 15 Feb 1993

Cruz A K, Titus R, Beverley S M

Abstract excerpt

We attempted to generate homozygous dhfr-ts (dihydrofolate reductase-thymidylate synthase) knockouts in virulent Leishmania major, an asexual diploid protozoan parasite. Transfection of a neo (neomycin phosphotransferase) targeting fragment yielded heterozygous replacement lines with high efficie...

Topics

Alleles
Aneuploidy
Animals
Blotting, Southern
Chromosomes
DNA, Protozoan
Diploidy
Kanamycin Kinase
Karyotyping
Leishmania
Phosphotransferases
Phosphotransferases (Alcohol Group Acceptor)
Polyploidy
Tetrahydrofolate Dehydrogenase
Thymidylate Synthase
Transfection
Virulence

Source

Open in PubMed Open full text

Identifiers

PMID: 8381972
DOI: 10.1073/pnas.90.4.1599
PMCID: PMC45922

Genes

DHFR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8381972-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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