Article
Formation of linear inverted repeat amplicons following targeting of an essential gene in Leishmania.
Nucleic acids research - 1 Jan 2005
Genest Paul-André, ter Riet Bas, Dumas Carole, Papadopoulou Barbara, van Luenen Henri G A M, Borst Piet
Abstract excerpt
Attempts to inactivate an essential gene in the protozoan parasite Leishmania have often led to the generation of extra copies of the wild-type alleles of the gene. In experiments with Leishmania tarentolae set up to disrupt the gene encoding the J-binding protein 1 (JBP1), a protein binding to the unusual base beta-D-glucosyl-hydroxymethyluracil (J) of Leishmania, we obtained JBP1 mutants containing linear DNA...
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