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Article

Role of the locus and of the resistance gene on gene amplification frequency in methotrexate resistant Leishmania tarentolae.

Nucleic acids research - 15 Sept 1999

Kündig C, Leblanc E, Papadopoulou B, Ouellette M

Abstract excerpt

The protozoan parasite Leishmania resists the antifolate methotrexate (MTX) by amplifying the R locus dihydrofolate reductase-thymidylate synthase ( dhfr-ts ) gene, the H locus ptr1 pterin reductase gene, and finally by mutation in a common folate/MTX transporter. Amplification of dhfr-ts has never been observed in Leishmania tarentolae MTX resistant mutants while ptr1 amplification is common. We have selected a...

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Identifiers

PMID: 10471733
DOI: 10.1093/nar/27.18.3653
PMCID: PMC148619

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Source receipt: pubmed, retrieved 7 Sept 2026.

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