Article
Role of the locus and of the resistance gene on gene amplification frequency in methotrexate resistant Leishmania tarentolae.
Nucleic acids research - 15 Sept 1999
Kündig C, Leblanc E, Papadopoulou B, Ouellette M
Abstract excerpt
The protozoan parasite Leishmania resists the antifolate methotrexate (MTX) by amplifying the R locus dihydrofolate reductase-thymidylate synthase ( dhfr-ts ) gene, the H locus ptr1 pterin reductase gene, and finally by mutation in a common folate/MTX transporter. Amplification of dhfr-ts has never been observed in Leishmania tarentolae MTX resistant mutants while ptr1 amplification is common. We have selected a...
Topics
- Alleles
- Animals
- Biological Transport
- Cell Line
- DNA, Protozoan
- DNA, Recombinant
- Drug Resistance
- Folic Acid Antagonists
- Gene Amplification
- Gene Dosage
- Genes, Protozoan
- Leishmania
- Methotrexate
- Multienzyme Complexes