Frequent amplification of a short chain dehydrogenase gene as part of circular and linear amplicons in methotrexate resistant Leishmania.

doi:10.1093/nar/21.18.4305

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Article

Frequent amplification of a short chain dehydrogenase gene as part of circular and linear amplicons in methotrexate resistant Leishmania.

Nucleic acids research - 11 Sept 1993

Papadopoulou B, Roy G, Ouellette M

Abstract excerpt

The H locus of Leishmania codes for a short chain dehydrogenase gene (ltdh) that is involved in antifolate resistance. Leishmania tarentolae cells, selected in a step by step fashion for resistance to the antifolate methotrexate (MTX), frequently amplified ltdh in response to drug selection. Both...

Topics

Animals
Biological Transport
Chromosomes
DNA, Circular
DNA, Protozoan
Drug Resistance
Gene Amplification
Gene Rearrangement
Leishmania
Methotrexate
Mutation
Oxidoreductases

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