Article
Overexpression of AP endonuclease protects Leishmania major cells against methotrexate induced DNA fragmentation and hydrogen peroxide.
Molecular and biochemical parasitology - 1 Jun 2005
Gallego Cláribel, Estévez Antonio M, Fárez Esther, Ruiz-Pérez Luis M, González-Pacanowska Dolores
Abstract excerpt
Generation of abasic (AP) sites is one of the main anomalies to arise in cellular DNA. These lesions are highly mutagenic, and need to be repaired by the base-excision repair (BER) system. Oxidative stress and misincorporation of dUTP are important sources of mutation load trough generation of AP sites. Kinetoplastid protozoa are able to survive in a highly oxidative environment within the host macrophages and...
Topics
- Animals
- DNA Fragmentation
- DNA Repair
- DNA-(Apurinic or Apyrimidinic Site) Lyase
- Hydrogen Peroxide
- Leishmania major
- Methotrexate
- Mutation
- Nucleic Acid Synthesis Inhibitors
- Oxidants