Article
Disruption of genes regulated during hematopoietic differentiation of mouse embryonic stem cells.
Developmental dynamics : an official publication of the American Association of Anatomists - 1 Jun 1998
Muth K, Bruyns R, Thorey I S, von Melchner H
Abstract excerpt
A retroviral gene trap vector (U3Tkneo) that selects for integrations in or near expressed 5' exons has been used to identify genes that are repressed during hematopoietic differentiation of mouse totipotent embryonic stem cells. The vector contains coding sequences for an HSV-thymidine kinase/neomycin phosphotransferase fusion protein in the U3 region of a Moloney murine leukemia virus LTR and allows selection...
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