Article
Gene trap screening as an effective approach for identification of Wnt-responsive genes in the mouse embryo.
Developmental dynamics : an official publication of the American Association of Anatomists - 1 Jun 2005
Yamaguchi Yoshifumi, Ogura Satoshi, Ishida Mio, Karasawa Mika, Takada Shinji
Abstract excerpt
In this study, we examined whether gene trap methodology, which would be available for systematic identification and functional analysis of genes, is effective for screening of Wnt-responsive genes during mouse development. We screened out two individual clones among 794 gene-trapped embryonic stem cell lines by their in vitro response to WNT-3A proteins. One gene was mainly expressed in the ductal epithelium of...
Topics
- Alleles
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- Down-Regulation
- Embryo, Mammalian
- Gene Expression Profiling
- Gene Expression Regulation, Developmental
- Genetic Vectors
- Intercellular Signaling Peptides and Proteins
- Kidney
- Mice