Article
A large-scale gene-trap screen for insertional mutations in developmentally regulated genes in mice.
Genetics - 1 Feb 1995
Wurst W, Rossant J, Prideaux V, Kownacka M, Joyner A, Hill D P, Guillemot F, Gasca S, Cado D, Auerbach A
Abstract excerpt
We have used a gene-trap vector and mouse embryonic stem (ES) cells to screen for insertional mutations in genes developmentally regulated at 8.5 days of embryogenesis (dpc). From 38,730 cell lines with vector insertions, 393 clonal integrations had disrupted active transcription units, as assaye...
Topics
- Animals
- Blastocyst
- Chimera
- Gene Expression Regulation, Developmental
- Genes, Reporter
- Genetic Testing
- Genetic Vectors
- Mice
- Mutagenesis, Insertional
- Mutation
- Stem Cells
- beta-Galactosidase