Article
Base pairing with U6atac snRNA is required for 5' splice site activation of U12-dependent introns in vivo.
RNA (New York, N.Y.) - 1 Jun 1998
Incorvaia R, Padgett R A
Abstract excerpt
The minor U12-dependent class of eukaryotic nuclear pre-mRNA introns is spliced by a distinct spliceosomal mechanism that requires the function of U11, U12, U5, U4atac, and U6atac snRNAs. Previous work has shown that U11 snRNA plays a role similar to U1 snRNA in the major class spliceosome by base pairing to the conserved 5' splice site sequence. Here we show that U6atac snRNA also base pairs to the 5' splice...
Topics
- Base Sequence
- Eukaryotic Cells
- Introns
- Models, Genetic
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- RNA Splicing
- RNA, Small Nuclear
- Suppression, Genetic