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Article

Mechanism for cryptic splice site activation during pre-mRNA splicing.

Proceedings of the National Academy of Sciences of the United States of America - 1 Aug 1990

Nelson K K, Green M R

Abstract excerpt

The 5' splice site of a pre-mRNA is recognized by U1 small nuclear ribonucleoprotein particles (snRNP) through base pairing with the 5' end of U1 small nuclear RNA (snRNA). Single-base substitutions within a 9-nucleotide 5'-splice-site sequence can abolish or attenuate use of that site and, in higher eukaryotes, can also activate nearby "cryptic" 5' splice sites. Here we show that the effects of single-base...

Topics

Animals
Base Sequence
Cell Line
Globins
Humans
Introns
Molecular Sequence Data
Mutation
RNA Precursors
RNA Splicing
Ribonucleoproteins
Ribonucleoproteins, Small Nuclear

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Thalassemia

Transcription, Genetic

Transfection

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Identifiers

PMID: 2143583
DOI: 10.1073/pnas.87.16.6253
PMCID: PMC54511

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2143583-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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