Article
Domains of human U4atac snRNA required for U12-dependent splicing in vivo.
Nucleic acids research - 1 Nov 2002
Shukla Girish C, Cole Andrea J, Dietrich Rosemary C, Padgett Richard A
Abstract excerpt
U4atac snRNA forms a base-paired complex with U6atac snRNA. Both snRNAs are required for the splicing of the minor U12-dependent class of eukaryotic nuclear introns. We have developed a new genetic suppression assay to investigate the in vivo roles of several regions of U4atac snRNA in U12-dependent splicing. We show that both the stem I and stem II regions, which have been proposed to pair with U6atac snRNA, are...
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