Article
The p53 gene in soft tissue sarcomas: prognostic value of DNA sequencing versus immunohistochemistry.
Anticancer research - 1 Jan 2000
Taubert H, Würl P, Bache M, Meye A, Berger D, Holzhausen H J, Hinze R, Schmidt H, Rath F W
Abstract excerpt
BACKGROUND: The aim of this study was to compare sequencing and immunohistochemistry (IHC) for their ability to detect p53 mutations and to assess their prognostic value in soft tissue sarcomas (STS). MATERIAL AND METHODS: 146 STS samples were investigated by single strand DNA conformation polymorphism (SSCP)-sequencing for p53 mutations. Additionally, IHC with five p53 antibodies (CM-1, Pab1801, Pab240, DO1,...
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